Game Changer in Life Science
Research & Develope
What is ELISA? (Enzyme-Linked Immunosorbent Assay): a method of quantifying the amount of antigenic protein contained in a biological sample using an antigen-antibody reaction.
It is a method of analyzing the amount of antigen by measuring the activity of an enzyme bound to an antibody.
Direct ELISA
This is a method of directly detecting an antigen using an antibody conjugated with an enzyme after the antigenic protein is first bound to the immunoplate.
Indirect ELISA
After the antigen is bound to the immunoplate, the antigen-specific primary detection antibody is bound to the antigen, and the primary antibody bound to the antigen is detected with the enzyme-bound secondary detection antibody.
Sandwich ELISA
This is a method of first binding an antigen-specific capture antibody to an immunoplate, then binding the antigen to the capture antibody, binding the primary detection antibody to the antigen, and then detecting the primary detection antibody with the secondary detection antibody, to which the enzyme is conjugated.
LFA(Lateral Flow Immunochromatographic Assay) based on Fluorescent